mmp9 promoter polymorphism (-1562 c/t) does not affect the serum levels of soluble micb and mica in breast cancer

background: the role of matrix metalloproteinase 9 (mmp9) in tumor invasion and progression is prominent. a single nucleotide polymorphism (snp) in the promoter region of mmp9 (-1562 c/t) increases the transcription and expression of this gene. on the other hand, mhc class i chain-related protein a and b (mica/b) in soluble forms may impair tumor immunogenicity by reducing natural killer group 2d (nkg2d) densities on nk cells and mmp9 enzyme activity has a prominent role in shedding of mica/b. objectives: to investigate the association between mmp9 (-1562 c/t) polymorphism and serum mica/b level in breast cancer patients. methods: in this case-control study, 105 patients with breast cancer and 100 healthy age-matched women were selected from yazd hospitals, iran. the polymorphism of mmp9 (-1562 c/t) was determined by pcr-rflp. concentration of micb and mica in the sera of breast cancer patients and healthy women were measured using elisa method. results: the frequency of cc, ct and tt genotypes and t allele of the mmp9 (-1562 c/t) did not show significant differences between breast cancer patients and healthy donors (p>0.05). on the other hand, the mean serum levels of micb and mica were significantly elevated in patients compared with healthy individuals (p